A Microfluidic Programmable Array for Label-free Detection of Biomolecules. By Peter Hume Dykstra. Abstract. One of the most promising ways to improve clinical diagnostic tools is to use microfluidic Lab-on-a-chip devices. Such devices can provide a dense array of fluidic components and sensors at the micro-scale which drastically reduce the necessary sample volumes and testing time. This.
We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.
A microfluidic streaming potential analyzer for DNA detection is constructed.. a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide.A method for detecting presence of a macromolecule of interest in a test droplet. A set of detection electrodes are provided in contact with a fluidic channel. The test droplet is provided in vicinity of the detection electrodes through the fluidic channel. An alternate current (AC) power at a first frequency is applied across the set of detection electrodes.An optical fiber sensor integrated microfluidic chip is presented for ultrasensitive detection of glucose. A long-period grating (LPG) inscribed in a small-diameter single-mode fiber (SDSMF) is employed as an optical refractive-index (RI) sensor. With the layer-by-layer (LbL) self-assembly technique, poly (ethylenimine) (PEI) and poly (acrylic acid) (PAA) multilayer film is deposited on the.
Sensitive and specific DNA biomarker detection is critical for accurately diagnosing a broad range of clinical conditions. However, the incorporation of such biosensing structures in integrated microfluidic devices is often complicated by the need for an additional labelling step to be implemented on the device. In this review we focused on presenting recent advances in label-free DNA.
Studying Electrotaxis in Microfluidic Devices,. Real-Time, Label-Free Detection of Biomolecular Interactions in Sandwich Assays by the Oblique-Incidence Reflectivity Difference Technique, Sensors 14 (12), 23307 (2014). (Special Issue on Advances in Optical Biosensors) 20. Yung-Shin Sun and Xiangdong Zhu. An Ellipsometry-Based Biosensor for Label-Free, Real-Time, and in-Situ Detection of DNA.
An increasing interest has been shown in microfluidic systems due to their properties including low consumption of reagents, short analysis time and easy integration. However, despite of these adva.
Microfluidic lab-on-chip devices coupled to tissue sampling using microdialysis provide an important new way for measuring real-time chemical changes as the low volume flow rates of microdialysis probes are ideally matched to the length scales of microfluidic devices. In this presentation, I will describe the combination of miniature electrochemical sensors and biosensors with 3D printed.
Lab-on-a-chip devices for electrochemical analysis of DNA hybridization events offer a technology for real-time and label-free assessment of biomarkers at the point-of-care. Here, we present a microfluidic lab-on-a-chip, with arrayed electrochemical sensors for the analysis of DNA hybridization events. A new dual layer microfluidic valved manipulation system is integrated providing controlled.
En microfluidic basert Elektrokjemisk biochip for Label-free DNA Hybridisering Analyse. Hadar Ben-Yoav 1, Peter H. Dykstra 1, Tanya Gordonov 2, William E. Bentley 2, Reza Ghodssi 1. 1 MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, 2 Institute for Bioscience and Biotechnology Research.
A label-free DNA hybridization biosensor based on side-hole-fiber integrated microfluidic devices has been proposed. Employing its birefringence adjustable feature, the hybridization detections of complementary matching DNA and single-base mutation DNA were achieved.
Integration of microfluidic technology with various detection methods such as optical (5, 6), electrochemical, and mechanical transduction have been employed for miniaturized POC devices. Among these detection methods, localized surface plasmon resonance (LSPR)-based optical biosensors have shown significant advantages of being fast, highly sensitive, simple, and providing high signal-to.
The popularity of this technique is mostlikely due to the simplicity with which microfluidic devices can be coupled to fluorescence excitation and detection schemes, as well as their ability to detect from low volume samples. There have been multiple examples of advances in detection using fluorescence-based methods as applied to microfluidic devices. Recent advances have included fluorescence.
In recent years, MEMS microfluidic devices based on polydimethylsiloxane (PDMS) polymer have been developed and integrated with the optical transducer to perform bio-particles detection devices (14-20). PDMS is one of the most widely used material to fabricate microfluidic devices, due to its biocompatibility and transparency from 240 to 1100 nm. In this work, a highly selective and sensitive.
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